IgA Monoclonal Antibody (mA-6E1), PE, eBioscience™, Invitrogen™

Description

• Description: The mA-6E1 monoclonal antibody reacts with the heavy chain of mouse IgA
• It does not react with other isotypes of antibody, including IgD, IgG or IgM, or with other species isotypes
• IgA, which is mainly found as a dimer through association of the J chain, is found in mucous secretions, but not in plasma
• Fluorochrome conjugated mA-6E1 is used as a detection secondary for mouse IgA primary antibodies
• Applications Reported: This mA-6E1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This mA-6E1 antibody has been tested by flow cytometric analysis as a second step to detect mouse IgA primary antibodies
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Mouse IgA is an immunoglobulin A antibody that is produced by B cells in response to an antigen
• It is a crucial component of the mucosal immune system, which protects the body from pathogens that enter through mucosal surfaces such as the respiratory, gastrointestinal, and urogenital tracts
• Mouse IgA is composed of two heavy chains and two light chains, and it is the most abundant immunoglobulin in mucosal secretions
• It plays a vital role in neutralizing pathogens and preventing their attachment to mucosal surfaces
• Mouse IgA is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.