CD85d (ILT4) Monoclonal Antibody (42D1), PE, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2
• It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family
• CD85d is a 110 kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs)
• Expression is confined to monocytes, macrophages and dendritic cells
• The ligand for CD85d is non-classical class I HLA-G, -B and G1
• Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM
• Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation
• Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection
• Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC
• Applications Reported: This 42D1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 42D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (1 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• LILRB2 (ILT4) gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4
• The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs)
• The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response
• It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity
• Multiple transcript variants encoding different isoforms have been found for this gene.