NOTCH3 Monoclonal Antibody (HMN3-133), PE, eBioscience™, Invitrogen™

Description

• Description: This HMN3-133 monoclonal antibody reacts with mouse Notch3, one of four members of the Notch family of receptors
• Notch receptors are 300-kDa single-pass transmembrane proteins
• While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling
• Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase
• The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription
• Notch 3 expression has been demonstrated on regulatory T cells and some thymocyte subsets, including CD4-CD8- and CD8SP cells
• In addition, this Notch receptor is prevalent in vascular smooth muscle and the central nervous system
• In addition to its role in stem cell hematopoiesis, Notch 3 plays a pivotal role in mammalian T cell lineage commitment and thymocyte development
• Specifically, Notch 3 has been implicated in the CD4-CD8- double negative (DN) to CD4+CD8+ double positive transition with greatest expression in DN3 (CD4-CD8-CD25+CD44-) cells
• Notch 3 may also be involved in regulating the pre-TCR checkpoint via crosstalk with the NFkappaB and E2A signaling pathways
• NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch
• In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development
• Homologues of the notch-ligands have also been identified in human
• NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination
• Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).