TdT Monoclonal Antibody (19-3), PE, eBioscience™, Invitrogen™

Description

• Description: This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes
• Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells
• Tdt plays a critical role during TCR and Ig gene rearrangement
• Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection
• Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells
• Applications Reported: This 19-3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 19-3 antibody has been tested by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (cat
• 00-5521) followed by flow cytometric analysis of mouse thymocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0
• Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template
• TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leuksemias
• The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt's lymphoma.