50-112-16

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 50-112-16-Each-of-1 In Stock ₹ 31,400.52

50-112-16 - Each of 1

₹ 31,400.52

In Stock

Quantity

1

Base Price: ₹ 31,400.52

GST (18%): ₹ 5,652.094

Total Price: ₹ 37,052.614

Antigen

SSEA1

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

eBioMC-480 (MC-480)

Conjugate

PE

Gene Alias

stage-specific embryonic antigen 1; CD15; SSEA-1; SSEA1; ELFT; FCT3A; FUC-TIV; FUTIV; LeX

Purification Method

Affinity chromatography

Regulatory Status

RUO

Target Species

Human, Mouse

Product Type

Antibody

Isotype

IgM

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Description

  • Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG)
  • No immunoreactivity is evident with undifferentiated human EC and ES cells
  • Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished
  • Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes
  • SSEA-1 is associated with cell adhesion, migration and differentiation
  • Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of the F9 cell line
  • This can be used at 5 µL (0.125 µg) per test
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determit (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g
  • granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells
  • There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x
  • Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction
  • This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells
  • Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins
  • CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

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Invitrogen

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Antigen:
SSEA1

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
eBioMC-480 (MC-480)

Conjugate:
PE

Gene Alias:
stage-specific embryonic antigen 1; CD15; SSEA-1; SSEA1; ELFT; FCT3A; FUC-TIV; FUTIV; LeX

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Target Species:
Human, Mouse

Product Type:
Antibody

Isotype:
IgM

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