CD122 Monoclonal Antibody (TU27), FITC, eBioscience™, Invitrogen™

Description

• Description: This TU27 monoclonal antibody reacts with human CD122, which is also known as interleukin-2 receptor beta (IL-2R beta)
• This receptor is expressed on NK cells, activated T cells, some B cells, monocytes, and T cell leukemias
• CD122 can also be detected during early NK cell commitment
• IL-2R beta associates with the IL-2R alpha (CD25) or IL-15R alpha and common gamma chain (CD132) to form the high affinity IL-2 and IL-15 receptors, respectively
• By itself, this membrane receptor can bind IL-2, but with intermediate affinity
• The binding of IL-2 results in internalization of the cytokine, tyrosine phosphorylation of IL-2Rbeta, and activation of signaling cascades such as the JAK/STAT pathway
• Applications Reported: This TU27 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This TU27 antibody has been pre-titrated and tested by flow cytometric analyis of normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms
• The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction
• The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer
• Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122
• The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein
• Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.