CD207 (Langerin) Monoclonal Antibody (eBioL31), eBioscience™, Invitrogen™

Description

• Description: The eBioL31 monoclonal antibody reacts with mouse CD207, also known as Langerin, which is expressed in a distinct subset of dendritic cells called Langerhans cells (LC)
• Mouse CD207 is a 48 kDa C-type lectin transmembrane protein that likely plays a role in antigen recognition and uptake
• LC are located in the epidermis and upon activation, reduce CD207 expression and begin migrating through the dermis towards lymphatic vessels
• Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules
• Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines
• Western Blotting with eBioL31 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse CD207 cDNA
• Preliminary data from mouse CD207 cDNA transfected cells suggests that eBioL31 can be used for intracellular flow cytometric analysis in combination with the Foxp3/Transcription Factor Staining Bufer Set (cat
• 00-5523)
• The epitope recognized by eBioL31 is in the extracellular domain within the CRD (carbohydrate recognition domain) and therefore can be used to detect both surface and intracellular langerin
• Differences in expression has been observed between mouse strains
• Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells
• Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD)
• Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules
• Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG)
• Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.