CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), PE, eBioscience™, Invitrogen™

Description

• Description: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein)
• Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region
• Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis
• TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1
• Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs
• Fn14 mRNA expression has been identified during liver regeneration
• It has been reported that ITEM-4 cross-reacts with mouse Fn14
• In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line
• The ITEM-4 antibody has been show to have blocking function
• Applications Reported: The ITEM-4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The ITEM-4 antibody is offered in 2 formats: - μg size: has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• TWEAK is a member of the TNF family and was first described as a weak inducer of apoptosis in demonstrate that this relatively small molecule is a fully transformed cell lines
• Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library
• TweakR is a small protein belonging to TNF receptor super family
• TweakR is capable of intiating a proliferative signal by binding to TRAF1, 2, and 3
• TweakR mRNA is upregulated during tissue regeneration in the aorta and liver
• It may also play a role in vascular cell migration and angiogenesis.