50-112-4027

CD45.2 Monoclonal Antibody (104), APC-eFluor™ 780, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 50-112-4027-Each-of-1 In Stock ₹ 12,748.44

50-112-4027 - Each of 1

₹ 12,748.44

In Stock

Quantity

1

Base Price: ₹ 12,748.44

GST (18%): ₹ 2,294.719

Total Price: ₹ 15,043.159

Antigen

CD45.2

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P06800

Gene Symbols

Ptprc

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

19264

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

104

Conjugate

APC-eFluor 780

Gene

Ptprc

Gene Alias

B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein

Host Species

Mouse

Quantity

25 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

Description

  • Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains
  • The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR
  • CD45.2 is expressed by all leukocytes in these strains
  • Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Light sensitivity: This tandem is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
  • CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
  • CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains
  • These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
  • Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

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Fischer Scientific

50-112-4027

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Antigen:
CD45.2

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P06800

Gene Symbols:
Ptprc

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
19264

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
104

Conjugate:
APC-eFluor 780

Gene:
Ptprc

Gene Alias:
B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein

Host Species:
Mouse

Quantity:
25 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

Img

Fischer Scientific

50-112-4030

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Antigen:
CD45

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P08575

Gene Symbols:
PTPRC

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
5788

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
HI30

Conjugate:
APC-eFluor 780

Gene:
PTPRC

Gene Alias:
B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

Host Species:
Mouse

Quantity:
25 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

Img

Fischer Scientific

50-112-4042

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Antigen:
CD117 (c-Kit)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P05532

Gene Symbols:
KIT

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
16590

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
ACK2

Conjugate:
APC-eFluor 780

Gene:
KIT

Gene Alias:
belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W

Host Species:
Rat

Quantity:
25 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2b κ

Img

Fischer Scientific

50-112-4043

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Antigen:
CD123

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P26951

Gene Symbols:
IL3RA

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
3563

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
6H6

Conjugate:
APC-eFluor 780

Gene:
IL3RA

Gene Alias:
CD123; CD123 antigen; CDw123; Cyrl; cytokine receptor-like protein CYRL; hIL-3Ra; Il-3 alpha subunit; IL-3 receptor alpha chain; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL3R; IL-3R subunit alpha; Il3ra; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor subunit alpha; interleukin 3 receptor, alpha; interleukin 3 receptor, alpha (low affinity); interleukin 3 receptor, alpha chain; interleukin-3 receptor class II alpha chain; Interleukin-3 receptor subunit alpha; Sut-1

Host Species:
Mouse

Quantity:
25 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ