CD1a Monoclonal Antibody (HI149), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells
• The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic
• Similar to MHC class I, CD1a associates with the beta2-microglobulin and is thought to play a role in antigen presentation
• Applications Reported: This HI149 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HI149 antibody has been pre-titrated and tested by flow cytometric analysis of MOLT-4 cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta 2 microglobulin
• CD1a is expressed by cortical thymocytes, Langerhan's cells and by interdigitating cells
• CD1a is also expressed by some malignancies of T cell lineage and in histiocytosis X
• CD1a is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues
• CD1a is structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin
• The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells
• The human genome contains five CD1 family genes organized in a cluster on chromosome 1
• The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands
• CD1a localizes to the plasma membrane and recycles vesicles of the early endocytic system
• At least five CD1 genes (CD1a, b, c, d, and e) are identified
• Alternatively spliced transcript variants of CD1a have been observed, but their biological validity have not been determined.