Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448
• mTOR is a serine/threonine protein kinase that functions as an ATP and amino acid sensor as well as to balance nutrient availability with cell growth, proliferation, motility, survival, protein synthesis, and transcription
• Activated mTOR increases production of enzymes necessary for glycolysis and controls the uptake of glucose and other nutrients
• Increased glucose uptake and metabolism helps fulfill the energy needs for mTOR-driven cell growth and proliferation
• When sufficient nutrients are available, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor, 4E-BP1
• mTOR is phosphorylated at S2448 via the PI3 kinase/Akt signaling pathway and is autophosphorylated at Ser2481
• Due to its critical role in regulation of cell growth, survival, and metabolism, and because it is often abnormally regulated in tumors, mTOR is under investigation as a potential target for anti-cancer therapy
• Applications Reported: This MRRBY antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This MRRBY antibody has been pre-titrated and tested by intracellular stining followed by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation
• Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer
• FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics
• Mutations affecting the gene results in Smith-Kingsmore syndrome.