CD11b/c Monoclonal Antibody (OX42), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This OX42 monoclonal antibody reacts with rat CD11b and CD11c
• CD11b, also known as integrin alpha M or Mac-1, is a component of complement receptor 3 (CR3)
• CD11c, also known as integrin alpha X, is a component of complement receptor 4 (CR4)
• CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells
• OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis
• The OX42 antibody has been reported to inhibit complement-mediated rosette formation by granulocytes and macrophages
• Moreover, studies demonstrate that OX42 can inhibit granulocyte aggregation
• Applications Reported: This OX42 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This OX42 antibody has been tested by flow cytometric analysis of rat splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain
• CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles
• It is identical to CR3, the receptor for the iC3b fragment of the third complement component
• CD11b is also a receptor for fibrogen, factor X and ICAM1
• It recognizes P1 and P2 peptides of fibrogen gamma chain
• The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes
• CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue
• CD11c encodes the integrin alpha X chain protein
• This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4)
• The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles
• Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.