CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin
• Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion
• These proteins share a common basic structure
• The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs
• The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins
• These catenins connect E-cadherin with the cytoskeleton
• Expression is found in most epidermal cells including melanocytes and kerotinocytes
• E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintece of tissue integrity
• Loss of E-cadherin function has been implicated in the progression of a variety of cancers
• E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided
• The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells
• Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This DECMA-1 antibody has been tested by flow cytometric analysis of MDCK cell line
• Optimal staining is achieved by intracellular staining as protein turnover can result in variable surface staining
• E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily
• The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer
• Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis
• The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization
• Identified transcript variants arise from mutation at consensus splice sites
• E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity
• In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16
• Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.