CD289 (TLR9) Monoclonal Antibody (eB72-1665), PE, eBioscience™, Invitrogen™

Description

• Description: eB72-1665 is generated against a portion of human toll-like receptor 9 (aa 273-288), a molecule reported to be expressed predomitly intracellularly
• TLR9 is a ∽115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA
• CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells
• When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice
• To date, at least twelve members of the Toll family have been identified
• This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor
• Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation
• TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9
• This may be due to limitations of antigen detection by flow cytometry
• Human pDCs matured in the presence of IL-3 have been reported to stain with eB72-1665 by immunofluorescence microscopy (Nat Immunol
• 5:190)
• Human Epithelial Cell lines were also reported to stain with this mAb (J
• Immunol
• 173: 1219)
• Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry
• Applications Reported: This eB72-1665 antibody has been reported for use in intracellular flow cytometric analysis
• Applications Tested: This eB72-1665 antibody has been tested by intracellular flow cytometric analysis of hTLR9 transfected cells
• This can be used at less than or equal to 1 µg per test
• A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity
• TLRs are highly conserved from Drosophila to humans and share structural and functional similarities
• They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity
• The various TLRs exhibit different patterns of expression
• This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes
• Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.