CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1)
• CD107a is a type I, lysosomal membrane protein that is extensively glycosylated
• It is expressed constitutively in the late endosomes-lysosomes in all cells
• CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells
• Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders
• Applications Reported: This eBio1D4B (1D4B) antibody has been reported for use in intracellular staining and flow cytometric analysis
• It has also been reported for use in surface staining in a flow cytometric based degranulation assay
• Applications Tested: This eBio1D4B (1D4B) antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-induced peritoneal exudate cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane
• LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail
• The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes
• LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes
• Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI)
• Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome
• Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation
• Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution
• LAMP1 is a good marker of mast cell activation.