CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule
• The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells
• CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells
• Applications Reported: This H35-17.2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This H35-17.2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system
• The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules
• The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain
• Both alpha and beta chains share significant homology to immunoglobulin variable light chains
• This gene encodes the CD8 beta chain isoforms
• Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described
• A pseudogene, also located on chromosome 2, has been identified.