CD14 Monoclonal Antibody (Sa2-8), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein
• CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS
• In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form
• Sa2-8 has weak antagonistic activity (in NF-kappaB activation or TNF alpha production with LPS stimulation)
• Applications Reported: This Sa2-8 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This Sa2-8 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils
• CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form
• CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein
• The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles
• Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes
• Alternative splicing results in multiple transcript variants encoding the same CD14 isoform
• Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.