BATF Monoclonal Antibody (MBM7C7), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The MBM7C7 monoclonal antibody reacts with human and mouse BATF (B cell activating transcription factor)
• BATF is a 14 kDa member of the AP-1 family of transcription factors and is involved in the repression of other AP-1 family members
• BATF is upregulated in activated T cells when compared to naive T and B cells and has been shown to be critical for Th17, but not Th1 or Th2 differentiation
• In Th17, BATF interacts with the promoters of IL17, IL17F, IL21 and IL22
• BATF has also been shown to be involved in CD8 T cell exhaustion in HIV infection
• Applications Reported: This MBM7C7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This MBM7C7 antibody has been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer Set (cat
• 00-5523) and protocol
• Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins)
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors
• The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins
• This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos
• BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense
• In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.