CD115 (c-fms) Monoclonal Antibody (AFS98), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1)
• CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells
• It is a 150 kDa c-fms gene product and belongs to immunoglobulin family
• CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage
• Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse BM derived macrophages
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias
• Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation
• CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases
• Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes
• Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes
• The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction
• Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.