50-112-9140
Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-9140-Each-of-1 | In Stock | ₹ 33,197.28 |
50-112-9140 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,197.28
GST (18%): ₹ 5,975.51
Total Price: ₹ 39,172.79
Antigen
Phospho-ZAP70/Syk (Tyr319, Tyr352)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P43403, P43404
Gene Symbols
ZAP70
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
22637, 7535
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
n3kobu5
Conjugate
PE
Gene
ZAP70
Gene Alias
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: This n3kobu5 monoclonal antibody recognizes human and mouse zeta chain-associated protein of 70 kD (also known as ZAP-70) and spleen tyrosine kinase (also known as SYK) when phosphorylated on Y319 and Y352, respectively
- ZAP-70 and SYK are members of the SYK protein tyrosine kinase (PTK) family that are phosphorylated and activated by Src family PTKs
- ZAP-70/SYK Y319/Y352 are located in the so-called interdomain of ZAP-70/SYK (between the N-terminal dual SH2 domains and the C-terminal kinase domain)
- Phosphorylation of ZAP-70 Y319 by Lck is necessary for T cell receptor (TCR)-dependent association of ZAP-70 with Lck and phospholipase C gamma and subsequent activation of calcium-dependent and Ras signaling cascades
- SYK Y352 phosphorylation by Fyn/Lyn is critical for propagation of B cell receptor (BCR) signaling and for B cell development
- Specificity of this n3kobu5 clone was determined by ELISA, flow cytometry, and western blotting
- Applications Reported: This n3kobu5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This n3kobu5 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells
- This can be used at 5 μL (0.03 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins
- ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation
- Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains
- Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
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Antigen: Phospho-ZAP70/Syk (Tyr319, Tyr352)
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: P43403, P43404
Gene Symbols: ZAP70
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 22637, 7535
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: n3kobu5
Conjugate: PE
Gene: ZAP70
Gene Alias: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Host Species: Mouse
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human, Mouse
Product Type: Antibody
Isotype: IgG2b κ
Antigen:
Phospho-ZAP70/Syk (Tyr319, Tyr352)
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
P43403, P43404
Gene Symbols:
ZAP70
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
22637, 7535
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
n3kobu5
Conjugate:
PE
Gene:
ZAP70
Gene Alias:
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Host Species:
Mouse
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Product Type:
Antibody
Isotype:
IgG2b κ
Antigen: CD32
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: P12318, P31994, P31995
Gene Symbols: FCGR2A, FCGR2B, FCGR2C
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 2212, 2213, 9103
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: 6C4 (CD32)
Conjugate: PerCP-eFluor 710
Gene: FCGR2B
Gene Alias: CD32; CD32 receptor 2; CD32 receptor 2a; CD32A; CD32B; CD32C; CDW32; Fc fragment of IgG receptor IIa; Fc fragment of IgG receptor IIb; Fc fragment of IgG receptor IIc (gene/pseudogene); Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIc, receptor for (CD32); Fc gamma receptor II; Fc gamma receptor IIa; Fc gamma receptor IIb; Fc gamma receptor IIC; Fc gamma RIIb; FCG2; Fc-gamma receptor II; fc-gamma RII; Fc-gamma RII-a; fc-gamma RII-b; Fc-gamma RII-c; fc-gamma-RIIa; Fc-gamma-RIIb; fc-gamma-RIIc; Fc-gamma-RII-D; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; fcRII; fcRII-a; fcRII-b; FCRIIC; FGFR2B; IGFR2; IgG Fc fragment receptor; IgG Fc gamma receptor II; igG Fc receptor II; IgG Fc receptor II-a; igG Fc receptor II-b; IgG Fc receptor II-c; Immunoglobulin G Fc receptor II; immunoglobulin G Fc receptor II-c; low affinity immunoglobulin gamma Fc region receptor II; Low affinity immunoglobulin gamma Fc region receptor II-a; low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c; MGC23887; MGC30032; RP11-5K23.6; soluble receptor; boFcRII-2
Host Species: Mouse
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
CD32
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
P12318, P31994, P31995
Gene Symbols:
FCGR2A, FCGR2B, FCGR2C
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
2212, 2213, 9103
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
6C4 (CD32)
Conjugate:
PerCP-eFluor 710
Gene:
FCGR2B
Gene Alias:
CD32; CD32 receptor 2; CD32 receptor 2a; CD32A; CD32B; CD32C; CDW32; Fc fragment of IgG receptor IIa; Fc fragment of IgG receptor IIb; Fc fragment of IgG receptor IIc (gene/pseudogene); Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIc, receptor for (CD32); Fc gamma receptor II; Fc gamma receptor IIa; Fc gamma receptor IIb; Fc gamma receptor IIC; Fc gamma RIIb; FCG2; Fc-gamma receptor II; fc-gamma RII; Fc-gamma RII-a; fc-gamma RII-b; Fc-gamma RII-c; fc-gamma-RIIa; Fc-gamma-RIIb; fc-gamma-RIIc; Fc-gamma-RII-D; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; fcRII; fcRII-a; fcRII-b; FCRIIC; FGFR2B; IGFR2; IgG Fc fragment receptor; IgG Fc gamma receptor II; igG Fc receptor II; IgG Fc receptor II-a; igG Fc receptor II-b; IgG Fc receptor II-c; Immunoglobulin G Fc receptor II; immunoglobulin G Fc receptor II-c; low affinity immunoglobulin gamma Fc region receptor II; Low affinity immunoglobulin gamma Fc region receptor II-a; low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c; MGC23887; MGC30032; RP11-5K23.6; soluble receptor; boFcRII-2
Host Species:
Mouse
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: HLA-A2
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: P04439
Gene Symbols: HLA-A
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 3105
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: BB7.2
Conjugate: PE-Cyanine7
Gene: HLA-A
Gene Alias: A29 protein; antigen presenting molecule; Aw-33; Aw-74; DAQB-90C11.16; FLJ26655; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA locus; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; Human leukocyte antigen A; leucocyte antigen A; leukocyte antigen class I-A; major histocompatibility complex, class I, A; MCH class I antigen; MHC 1; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; MHC1; truncated MHC class I antigen
Host Species: Mouse
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG2b κ
Antigen:
HLA-A2
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
P04439
Gene Symbols:
HLA-A
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
3105
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
BB7.2
Conjugate:
PE-Cyanine7
Gene:
HLA-A
Gene Alias:
A29 protein; antigen presenting molecule; Aw-33; Aw-74; DAQB-90C11.16; FLJ26655; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA locus; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; Human leukocyte antigen A; leucocyte antigen A; leukocyte antigen class I-A; major histocompatibility complex, class I, A; MCH class I antigen; MHC 1; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; MHC1; truncated MHC class I antigen
Host Species:
Mouse
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG2b κ
Antigen: CD150
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: Q9QUM4
Gene Symbols: Slamf1
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 27218
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: mShad150
Conjugate: FITC
Gene: Slamf1
Gene Alias: 4933415F16; AA177906; CD150; CD150 antigen; CDw150; ESTM51; IPO-3; RGD1560634; signaling lymphocyte activation molecule; signaling lymphocytic activation molecule; signaling lymphocytic activation molecule family member 1; Slam; SLAM family member 1; Slamf1
Host Species: Rat
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Mouse
Product Type: Antibody
Isotype: IgG2b λ
Antigen:
CD150
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
Q9QUM4
Gene Symbols:
Slamf1
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
27218
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
mShad150
Conjugate:
FITC
Gene:
Slamf1
Gene Alias:
4933415F16; AA177906; CD150; CD150 antigen; CDw150; ESTM51; IPO-3; RGD1560634; signaling lymphocyte activation molecule; signaling lymphocytic activation molecule; signaling lymphocytic activation molecule family member 1; Slam; SLAM family member 1; Slamf1
Host Species:
Rat
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Mouse
Product Type:
Antibody
Isotype:
IgG2b λ