CD326 (EpCAM) Monoclonal Antibody (MH99), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody MH99 recognizes human CD326 also known as EpCAM (Epithelial cell adhesion molecule)
• This 40 kDa type I transmembrane protein is involved in cell-cell interactions in a calcium-independent manner
• EpCAM is expressed primarily on the basolateral surface of most epithelia
• Although normal epithelia express low levels of CD326, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype
• CD326 has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM
• Based on crossblocking studies, MH99 and 1B7 see overlapping epitopes
• Applications Reported: This MH99 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MH99 antibody has been pre-titrated and tested by flow cytometric analysis of the A549 cell line
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule
• Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E
• Ep-CAM mediates Ca2+-independent homotypic interactions
• Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells
• Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation
• Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage
• Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types
• Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.