CD28 Monoclonal Antibody (1C6), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The 1C6 monoclonal antibody reacts with canine CD28, which is expressed by thymocytes, a majority of T cells, and a subset of NK cells
• CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells
• Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells
• The 1C6 antibody has been reported to have antagonist activity, as well as induce proliferation
• The 1C6 and 5B8 antibodies recognize different epitopes of canine CD28
• Applications Reported: This 1C6 antibody has been reported for use in flow cytometric analysis and functional assays
• Proliferation assays with the 1C6 antibody are best performed using polypropylene plates
• (Fluorochrome-conjugated 5B8 is recommended for use in flow cytometry.)
• Applications Tested: This 1C6 antibody has been tested by flow cytometric analysis of normal canine peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
• Storage and handling: Use in a sterile environment
• Filtration: 0
• CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein
• CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes
• Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells
• In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death
• In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28
• In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR
• The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells
• Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.