CD335 (NKp46) Monoclonal Antibody (9E2), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This 9E2 monoclonal antibody reacts with human CD335, which is also known as NKp46
• Expressed on NK cells, CD335 is a member of the natural cytotoxicity receptor family that also includes NKp30 and NKp44
• This type I transmembrane glycoprotein is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor and virus-infected (e.g., influenza) cells
• CD335 also interacts with pancreatic beta cells during the development of type I diabetes
• The 9E2 antibody has been reported to induce cytotoxicity
• Applications Reported: This 9E2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 9E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis
• NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).