HLA-DQ Monoclonal Antibody (SK10), APC, eBioscience™, Invitrogen™

Description

• Description: This SK10 monoclonal antibody reacts with the human MHC Class II HLA-DQ molecule
• This cell surface receptor is a heterodimeric protein composed of an alpha (HLA-DQA1) and beta (HLA-DQB1) subunit
• HLA-DQ is expressed on antigen presenting cells such as B cells, monocytes, macrophages, dendritic cells, and activated T cells
• HLA-DQ plays a role in presenting peptide antigens to CD4+ T lymphocytes
• Reports indicate that certain alleles of HLA-DQ are associated with celiac disease and type I diabetes
• Applications Reported: This SK10 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SK10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• HLA-DQ is one of the HLA (human leukocyte antigen) molecules belonging to class II of the major histocompatibility protein (MHC) surface proteins
• HLA-DQ and the other MHC class II molecules are heterodimeric proteins consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane
• They play a central role in the immune system by presenting peptides derived from extracellular proteins
• MHC Class II molecules are expressed in antigen-presenting cells (APC: B lymphocytes, dendritic cells, macrophages) and present peptides endocytosed, digested, and loaded onto the proteins prior to their migration to the cell surface
• Other human MHC class II molecules are HLA-DP, HLA-DM, HLA-DR, HLA-DOA, and HLA-DOB
• HLA-DQ displays significant variability, particularly in the beta subunit within the region that affects the peptides bound and presented by the protein
• Different classes of DQ based on similar beta subunits allow for the grouping of these variants into serotypes, which can often be recognized by antibodies raised against these subunits
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.