50-113-0714

Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), eFluor™ 570, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

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Pack Size SKU Availability Price
Each of 1 50-113-0714-Each-of-1 In Stock ₹ 36,790.80

50-113-0714 - Each of 1

₹ 36,790.80

In Stock

Quantity

1

Base Price: ₹ 36,790.80

GST (18%): ₹ 6,622.344

Total Price: ₹ 43,413.144

Antigen

Phospho-Histone H2A.X (Ser139)

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P16104, P27661

Gene Symbols

H2AX

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

15270, 3014

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot

Clone

CR55T33

Conjugate

eFluor 570

Gene

H2AX

Gene Alias

AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human, Mouse

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX
  • H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin
  • Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4
  • After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK) are activated to phosphorylate serine 139 in H2AX
  • This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair
  • The monoclonal antibody CR55T33 recognizes a single band of approximately 15 kDa on reduced cell lysates from Jurkat cells stimulated with etoposide
  • Applications Reported: This CR55T33 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry
  • Applications Tested: This CR55T33 antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 1 μg/mL
  • Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle
  • In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX
  • The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs
  • The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner
  • Structurally, H2A.x contains 143 amino acid residues
  • Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs
  • There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA
  • The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA
  • Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome
  • Histone H2A.x contributes to histone-formation and therefore the structure of DNA
  • Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

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Invitrogen

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Antigen:
Phospho-Histone H2A.X (Ser139)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P16104, P27661

Gene Symbols:
H2AX

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
15270, 3014

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot

Clone:
CR55T33

Conjugate:
eFluor 570

Gene:
H2AX

Gene Alias:
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329

Host Species:
Mouse

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Human, Mouse

Product Type:
Antibody

Isotype:
IgG1 κ

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Fischer Scientific

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Antigen:
__

Classification:
__

Concentration:
0.2 mg/mL

Formulation:
__

Gene Accession No.:
__

Gene Symbols:
__

Purification Method:
__

Regulatory Status:
__

Gene ID (Entrez):
__

Content And Storage:
__

Form:
Liquid

Applications:
__

Clone:
__

Conjugate:
eFluor 615

Gene:
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Gene Alias:
__

Host Species:
__

Quantity:
100 μg

Primary or Secondary:
__

Target Species:
__

Product Type:
Streptavidin Conjugate (fluorescent)

Isotype:
__

Img

Fischer Scientific

50-113-0719

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Antigen:
Ki-67

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
E9PVX6, P46013

Gene Symbols:
Mki67

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
100686578, 102135895, 17345, 291234, 4288

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Immunocytochemistry, Immunohistochemistry (Frozen)

Clone:
SolA15

Conjugate:
eFluor 615

Gene:
Mki67

Gene Alias:
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

Host Species:
Rat

Quantity:
25 μg

Primary or Secondary:
Primary

Target Species:
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat

Product Type:
Antibody

Isotype:
IgG2a κ

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Antigen:
Cytokeratin 19

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P08727

Gene Symbols:
KRT19

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
3880

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin)

Clone:
BA17

Conjugate:
eFluor 615

Gene:
KRT19

Gene Alias:
40-kDa keratin intermediate filament; AI663979; C K1CS; CK 19; CK19; CK-19; cytokeratin 19; cytokeratin-19; EndoC; K19; K1CS; Ka19; keratin 19; keratin 19, type I; keratin complex 1, acidic, gene 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt-1.19; Krt1-19; Krt19; LOC100344434; MGC15366; Type I keratin Ka19

Host Species:
Mouse

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ