CD103 (Integrin alpha E) Monoclonal Antibody (Ber-ACT8), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The Ber-ACT8 monoclonal antibody reacts with human CD103, the alpha E integrin
• CD103 non-covalently associates with integrin beta7
• CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes
• CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias
• In vitro stimulation of human T cells with mitogens induces upregulation of CD103
• Epithelial cell antigen (E-cadherin) binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium
• Cross-blocking studies suggest that B-Ly7 and Ber-ACT8 see different epitopes
• Applications Reported: This Ber-ACT8 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This Ber-ACT8 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells
• In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain
• CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs
• CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes
• CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain
• ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains
• In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen
• CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation
• Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.