CD249 (BP-1) Monoclonal Antibody (6C3), Biotin, eBioscience™, Invitrogen™

Description

• Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen
• BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer
• This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells
• The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7
• Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system
• Probably plays a role in regulating growth and differentiation of early B-lineage cells.