IL-4 Monoclonal Antibody (MP4-25D2), Biotin, eBioscience™, Invitrogen™

Description

• Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells
• Applications Reported: The MP4-25D2 antibody has been reported for use in detection of human IL-4 by ELISA and for intracellular staining for flow cytometry
• Applications Tested: The MP4-25D2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the purified 8D4-8 (14-7049) antibody for capture and recombinant human IL-4 (14-8049) as the standard
• A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL
• A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate
• Filtration: 0.2 μm post-manufacturing filtered
• Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor
• IL-4 has been found to have the broadest range of actions
• The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13
• STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4
• The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q
• In particular, IL-4 is located in close proximity to IL13 on the chromosome
• Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome
• IL-4 delta 2 is a known splice variant of IL-4.