GM-CSF Monoclonal Antibody (MP1-31G6), Biotin, eBioscience™, Invitrogen™

Description

• Description: The MP1-31G6 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF), a 14 kDa cytokine secreted by activated T cells and macrophages
• Applications Reported: MP1-31G6 has been reported for use in detection of mouse GM-CSF by ELISA
• Applications Tested: The MP1-31G6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the purified MP1-22E9 (14-7331) antibody for capture and recombinant mouse GM-CSF (14-8331) as the standard
• A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL
• A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate
• Filtration: 0.2 μm post-manufacturing filtered
• The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes
• The active form of the protein exists as a homodimer in the extracellular space
• This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia
• Other genes in the cluster include interleukins 4, 5, and 13
• The gene is involved in promoting tissue inflammation
• Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome
• Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.