CD1d Monoclonal Antibody (1B1), eBioscience™, Invitrogen™

Description

• Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules
• While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II
• 1B1 detects CD1d at varying levels on mouse leukocytes
• 1B1 detects beta2m associated CD1d
• Applications Reported: The 1B1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining
• 1B1 has also been reported in in vivo and in vitro studies
• (Please use Functional Grade purified 1B1, cat
• 16-0011, in functional assays.)
• Applications Tested: The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes
• Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells
• CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins
• The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin
• The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells
• The human genome contains five CD1 family genes organized in a cluster on chromosome 1
• The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands
• The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail
• Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.