CD38 Monoclonal Antibody (HIT2), eBioscience™, Invitrogen™

Description

• Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein
• CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes
• CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity
• Applications Reported: The HIT2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining
• Applications Tested: The HIT2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• The HIT2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 40 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells
• CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor
• Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e
• identification of myelomas), and as a marker for activated B and T cells
• CD38 participates in cell adhesion, signal transduction and calcium signaling
• Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma
• Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.