CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), eBioscience™, Invitrogen™

Description

• Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3
• CD51, an ∽120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components
• Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets
• The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin
• Applications Reported: The 23C6 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections
• 23C6 has also been reported in blocking of some adhesive processes
• (Please use Functional Grade purified 23C6 in functional assays.)
• Applications Tested: The 23C6 antibody has been tested by flow cytometric analysis of human melanoma cell line and peripheral blood
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex
• This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen
• Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.