CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™, Invitrogen™

Description

• Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3
• CD61 is expressed by activated T cells, granulocytes, and platelet
• CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa
• These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor
• Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections
• 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes
• (Please use Functional Grade purified 2C9.G3, cat
• 16-0611, in functional assays.)
• Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors
• CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin
• CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface
• CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51
• CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin
• The CD61 protein product is composed of an alpha chain and a beta chain
• A given chain may combine with multiple partners resulting in different integrins
• CD61 is found along with the alpha IIb chain in platelets
• Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling
• Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.