CD105 (Endoglin) Monoclonal Antibody (MJ7/18), eBioscience™, Invitrogen™

Description

• Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin
• This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells
• It is suggested that CD105 functions in adhesion and embryonic angiogenesis
• Applications Reported: The MJ7/18 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections
• Applications Tested: The MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells or mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells
• It exists on an O- and N-glycosylated homodimer
• Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker
• CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.