CD124 Monoclonal Antibody (X2/45-12), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody X2/45-12 reacts with human CD124, which is also known as the IL-4R alpha chain
• CD124 can associate with the common gamma chain to form the type I IL-4R or with IL-13Ralpha1 to form the type II IL-4R
• While both receptor types can bind IL-4, only the type II receptor can associate with IL-13
• The type I IL-4 receptor is found on T, B, NK, mast cells, basophils, and macrophages
• The type II IL-4 receptor is found on many non-hematopoietic cells and macrophages
• CD124 contains an ITIM that associates with and activates STAT6
• IL-4 is responsible for the generation of high-affinity IgE antibodies and driving the polarization of naive Th0 cells into Th2 cells
• In mucosal barriers, the cytokine is involved in the recruitment of innate and adaptive immune effector cells and mucous overproduction
• Finally, IL-4R also exists as a soluble form that serves primarily as a carrier protein for IL-4 that increases its half-life
• The X2/45 antibody has been reported to have antagonist activity
• Applications Reported: This X2/45-12 antibody has been reported for use in flow cytometric analysis, and western blotting
• (Please use Functional Grade purified X2/45-12, cat
• 16-1249, in functional assays.)
• Applications Tested: This X2/45-12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation
• The IL4 response is involved in promoting Th2 differentiation
• The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells
• It has no signaling abilities
• Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.