CD171 Monoclonal Antibody (eBio5G3 (5G3)), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody eBio5G3 recognizes CD171 also known as neural cell adhesion molecule L1
• CD171 is a member of the Ig superfamily containing 6 extracellular Ig domains and five fibronectin type III-like repeats
• CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration
• Expression of CD171 has been found on monocytes and mature monocytic-derived and follicular DCs, a minor subset of lymphocytes in addition to that found on neuronal tissue and some tumor cells lines
• Expression of CD171 on tumors is thought to contribute to tumor progression
• Epitope of eBio5G3 is in amino-terminal Ig-like domain
• Applications Reported: This eBio5G3 (5G3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, and immunohistology staining of frozen tissue sections
• Applications Tested: This eBio5G3 (5G3) antibody has been tested by flow cytometric of tumor cell line Panc-1
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family
• The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain
• This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation
• Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus)
• Alternative splicing of a neuron-specific exon is thought to be functionally relevant.