GILZ Monoclonal Antibody (CFMKG15), eBioscience™, Invitrogen™

Description

• Description: GILZ is a leucine-zipper-containing protein that is induced by glucocorticoids
• It is expressed in mast cells, monocytes, macrophages, dendritic cells, and T cells
• GILZ has been shown to inhibit production of IL-2 as well as TCR-driven upregulation of FasL
• GILZ mediates these changes in transcription, at least in part, by inhibiting DNA-binding of the transcription factors AP-1 and NF-kappaB
• GILZ has also been shown to mediate anti-inflammatory signals in macrophages and dendritic cells by interfering with NF-kappaB-mediated signaling
• Although GILZ has been reported to localize to the nucleus, more recent reports as well as testing at eBioscience have observed predomitly cytoplasmic staining
• Applications Reported: This CFMKG15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoblotting (WB), and immunocytochemistry
• (Fluorochrome conjugated CFMKG15 is recommended for use in intracellular flow cytometry.)
• Applications Tested: This CFMKG15 antibody has been tested by western blot analysis of dexamethasone-treated mouse thymocytes
• CFMKG15 recognizes endogenous mouse GILZ with an approximate molecular weight of 14 kDa; a non-specific band of approximately 95 kDa is also observed
• CFMKG15 also recognizes endogenous human GILZ in dexamethasone-treated peripheral blood mononuclear cells, with an approximate molecular weight of 14 kDa; non-specific bands may also appear at approximately 33 kDa and 95 kDa
• The TSC22 domain family member 3 protein (TSC22D3) is a leucine zipper protein that functions as a transcriptional regulator
• The expression of TSC22D3 is stimulated by glucocorticoids and IL-10 and is thought to play a key role in the anti-inflammatory and immunosuppressive effects of these molecules
• TSC22D3 can physically interact with and inhibit the activities of key inflammatory signaling mediators such NF-Kappa-B and AP-1
• TSC22D3 functions as a transcriptional co-activator for various nuclear receptors and NF-Kappa-B
• It has also been shown to be involved in the differentiation of mesenchymal stem cells towards osteoblasts and bone formation.