50-131-34
Nanog Monoclonal Antibody (eBioMLC-51), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-131-34-Each-of-1 | In Stock | ₹ 15,315.24 |
50-131-34 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,315.24
GST (18%): ₹ 2,756.743
Total Price: ₹ 18,071.983
Antigen
Nanog
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
Q80Z64
Gene Symbols
NANOG
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
71950
Content And Storage
4° C
Form
Liquid
Applications
Immunocytochemistry, Western Blot
Clone
eBioMLC-51
Conjugate
Unconjugated
Gene
NANOG
Gene Alias
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
Host Species
Rat
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
Description
- Description: The eBioMLC51 monoclonal antibody recognizes mouse og
- og is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3
- Expression of og in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells
- og expression often overlaps, but is not identical to, that of Oct4
- og is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells
- og acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus
- The CD2 domain is unique to og, whereas the NK2 DNA binding domain is well conserved
- Immunoblotting using eBioMLC51 reveals a band at ∽ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen
- Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat
- 00-5523, for intracellular staining of mouse og
- Applications Reported: This eBioMLC-51 antibody has been reported for use in western blotting, and immunocytochemistry
- Applications Tested: This eBioMLC-51 antibody has been tested by immunoblotting the F9 cell line or by immunocytochemistry of fixed and permeabilized F9 cells
- NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells
- NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells
- Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain
- Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2
- Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst
- Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line
- Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal
- The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases
- Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts
- Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine
- When overexpressed, NANOG promotes cells to enter into S phase and proliferation
- Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
Compare Similar Items
Show Difference
Antigen: Nanog
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: Q80Z64
Gene Symbols: NANOG
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 71950
Content And Storage: 4° C
Form: Liquid
Applications: Immunocytochemistry, Western Blot
Clone: eBioMLC-51
Conjugate: Unconjugated
Gene: NANOG
Gene Alias: 2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
Host Species: Rat
Quantity: 25 μg
Primary or Secondary: Primary
Target Species: Mouse
Product Type: Antibody
Isotype: IgG2a κ
Antigen:
Nanog
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
Q80Z64
Gene Symbols:
NANOG
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
71950
Content And Storage:
4° C
Form:
Liquid
Applications:
Immunocytochemistry, Western Blot
Clone:
eBioMLC-51
Conjugate:
Unconjugated
Gene:
NANOG
Gene Alias:
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
Host Species:
Rat
Quantity:
25 μg
Primary or Secondary:
Primary
Target Species:
Mouse
Product Type:
Antibody
Isotype:
IgG2a κ
Antigen: Nanog
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: Q9H9S0
Gene Symbols: NANOG
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 79923
Content And Storage: 4° C
Form: Liquid
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone: hNanog.2
Conjugate: Unconjugated
Gene: NANOG
Gene Alias: 2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
Host Species: Mouse
Quantity: 25 μg
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
Nanog
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
Q9H9S0
Gene Symbols:
NANOG
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
79923
Content And Storage:
4° C
Form:
Liquid
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone:
hNanog.2
Conjugate:
Unconjugated
Gene:
NANOG
Gene Alias:
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
Host Species:
Mouse
Quantity:
25 μg
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: Nanog
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: Q9H9S0
Gene Symbols: NANOG
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 79923
Content And Storage: 4° C
Form: Liquid
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone: hNanog.2
Conjugate: Unconjugated
Gene: NANOG
Gene Alias: 2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
Nanog
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
Q9H9S0
Gene Symbols:
NANOG
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
79923
Content And Storage:
4° C
Form:
Liquid
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone:
hNanog.2
Conjugate:
Unconjugated
Gene:
NANOG
Gene Alias:
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: NOTCH1
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P46531, Q01705
Gene Symbols: Notch1
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 18128, 4851
Content And Storage: 4° C
Form: Liquid
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Clone: mN1A
Conjugate: Unconjugated
Gene: Notch1
Gene Alias: 9930111A19Rik; AOS5; AOVD1; Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila); hN1; lin-12; major type A protein; Mis6; Motch; Motch A; mT14; N1; neurogenic locus notch homolog protein 1; NEXT; NICD; NOTCH; notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; notch receptor 1; Notch1; p300; RP23-306D20.12; RP23-306D20.12-001; Tan1; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
Host Species: Mouse
Quantity: 50 μg
Primary or Secondary: Primary
Target Species: Human, Mouse
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
NOTCH1
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P46531, Q01705
Gene Symbols:
Notch1
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
18128, 4851
Content And Storage:
4° C
Form:
Liquid
Applications:
Flow Cytometry, Immunohistochemistry, Western Blot
Clone:
mN1A
Conjugate:
Unconjugated
Gene:
Notch1
Gene Alias:
9930111A19Rik; AOS5; AOVD1; Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila); hN1; lin-12; major type A protein; Mis6; Motch; Motch A; mT14; N1; neurogenic locus notch homolog protein 1; NEXT; NICD; NOTCH; notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; notch receptor 1; Notch1; p300; RP23-306D20.12; RP23-306D20.12-001; Tan1; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
Host Species:
Mouse
Quantity:
50 μg
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Product Type:
Antibody
Isotype:
IgG1 κ