50-133-99

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 50-133-99-Each-of-1 In Stock ₹ 23,785.68

50-133-99 - Each of 1

₹ 23,785.68

In Stock

Quantity

1

Base Price: ₹ 23,785.68

GST (18%): ₹ 4,281.422

Total Price: ₹ 28,067.102

Antigen

Ly-6A/E (Sca-1)

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P05533, Q64253

Gene Symbols

Ly6a, Ly6e

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

110454, 17069

Content And Storage

4° C

Form

Liquid

Applications

Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot

Clone

D7

Conjugate

Unconjugated

Gene

Ly6a

Gene Alias

9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1

Host Species

Rat

Quantity

500 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

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Description

  • Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins
  • D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells
  • Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others
  • Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype
  • The Ly-6 family is involved in regulation and function of T cell activation
  • Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset
  • Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting
  • Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • (Fluorochrome-conjugated D7 is recommended for use in flow cytometry.)
  • Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family
  • It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice
  • Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC
  • Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen
  • Sca-1 may be involved in the regulation of B and T cell activation.

Compare Similar Items

Show Difference

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Fischer Scientific

50-133-99

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Antigen:
Ly-6A/E (Sca-1)

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P05533, Q64253

Gene Symbols:
Ly6a, Ly6e

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
110454, 17069

Content And Storage:
4° C

Form:
Liquid

Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot

Clone:
D7

Conjugate:
Unconjugated

Gene:
Ly6a

Gene Alias:
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1

Host Species:
Rat

Quantity:
500 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

Img

Invitrogen

50-134-09

--


Antigen:
CD273 (B7-DC)

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q9WUL5

Gene Symbols:
PDCD1LG2

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
58205

Content And Storage:
4° C

Form:
Liquid

Applications:
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization, Western Blot

Clone:
TY25

Conjugate:
Unconjugated

Gene:
PDCD1LG2

Gene Alias:
B7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; butyrophilin-like protein; CD273; F730015O22Rik; MGC124039; MGC124040; PD1 ligand 2; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; Pdcd1lg2; Pdl2; PD-L2; Programmed cell death 1 ligand 2; programmed death ligand 2

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

Img

Fischer Scientific

50-134-12

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Antigen:
CD202b (TIE2)

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q02858

Gene Symbols:
TEK

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
21687

Content And Storage:
4° C

Form:
Liquid

Applications:
Flow Cytometry, Immunohistochemistry (Frozen)

Clone:
TEK4

Conjugate:
Unconjugated

Gene:
TEK

Gene Alias:
AA517024; Angiopoietin-1 receptor; CD202B; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; endothelial-specific receptor tyrosine kinase; endothelium-specific receptor tyrosine kinase 2; endothelium-specific receptor tyrosine kinase Tie-2; hTIE2; Hyk; mTIE2; p140 TEK; protein-tyrosine kinase Tie2; STK1; Tek; TEK receptor tyrosine kinase; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tie2; tie-2; tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2; tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor Tie-2; VMCM; VMCM1

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG1 κ

Img

Fischer Scientific

50-134-13

--


Antigen:
CD202b (TIE2)

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q02858

Gene Symbols:
TEK

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
21687

Content And Storage:
4° C

Form:
Liquid

Applications:
Flow Cytometry, Immunohistochemistry (Frozen)

Clone:
TEK4

Conjugate:
Unconjugated

Gene:
TEK

Gene Alias:
AA517024; Angiopoietin-1 receptor; CD202B; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; endothelial-specific receptor tyrosine kinase; endothelium-specific receptor tyrosine kinase 2; endothelium-specific receptor tyrosine kinase Tie-2; hTIE2; Hyk; mTIE2; p140 TEK; protein-tyrosine kinase Tie2; STK1; Tek; TEK receptor tyrosine kinase; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tie2; tie-2; tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2; tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor Tie-2; VMCM; VMCM1

Host Species:
Rat

Quantity:
500 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG1 κ