Ub-K63 Monoclonal Antibody (HWA4C4), eBioscience™, Invitrogen™

Description

• Description: Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms
• Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation
• The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63)
• K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed
• In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair
• There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy
• HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin
• It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains
• Applications Reported: This HWA4C4 antibody has been reported for use in immunoblotting (WB)
• Applications Tested: This HWA2-8°C4 antibody has been tested by immunoblot of lysine-63-linked polyubiquitin chains
• This can be used at less than or equal to 2 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications
• The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation
• Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3
• The first reaction involves ubiquitin-activating enzyme
• The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.