beta Galactosidase Fusion Protein Polyclonal Antibody, eBioscience™, Invitrogen™

Description

• Description: The rabbit polyclonal antibody reacts with bBeta galactosidase
• Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions
• The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene
• The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells
• The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope
• Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining
• Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB)
• (1:400 starting dilution)
• It is recommended that this antibody be titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins
• Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme
• This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates
• Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome
• Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.