IL-13 Monoclonal Antibody (PVM13-1), eBioscience™, Invitrogen™

Description

• Description: The PVM13-1 antibody reacts with human interleukin-13 (IL-13)
• IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8^+ T cells, and NK cells
• The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression
• Applications Reported: PVM13-1 has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, and ELISA
• (Fluorochrome conjugated PVM13-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified PVM13-1, cat
• 16-7139, in functional assays.)
• Applications Tested: The PVM13-1 antibody has been tested as the capture antibody in a sandwich ELISA and ELISPOT assay for analysis of human interleukin-13 (IL-13) in combination with the biotinylated anti-human IL-13 polyclonal antibody (13-7138) antibody for detection and recombinant human IL-13 (14-8139) as the standard
• A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
• A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses
• IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils
• IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells
• Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF
• The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.