50-136-76
IFN gamma Monoclonal Antibody (DB-1 (DB1)), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-136-76-Each-of-1 | In Stock | ₹ 28,234.80 |
50-136-76 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 28,234.80
GST (18%): ₹ 5,082.264
Total Price: ₹ 33,317.064
Antigen
IFN gamma
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P01581
Gene Symbols
IFNG
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
25712
Content And Storage
4° C
Form
Liquid
Applications
ELISA
Clone
DB-1 (DB1)
Conjugate
Unconjugated
Gene
IFNG
Gene Alias
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
Host Species
Mouse
Quantity
500 μg
Primary or Secondary
Primary
Target Species
Rat
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The DB-1 monoclonal antibody reacts with rat interferon-gamma (IFN-gamma), a 15-20 kDa cytokine secreted by Th1 cells
- For optimal detection of rat IFN-gamma by sandwich ELISA, a combination of purified DB-1 for capture and biotinylated polyclonal anti-rat IFN-gamma for detection is recommended
- DB-1 is used for capture of rat IFN-gamma by ELISA
- Applications Reported: DB-1 has been reported for use in capture of rat IFN gamma by ELISA
- Applications Tested: The DB-1 (DB1) antibody has been tested as the capture antibody in a sandwich ELISA for analysis of rat IFNg (Interferon-gamma, IFN-g) in combination with the biotin anti-rat IFNg poly (13-7320) antibody for detection and recombinant rat IFNg (14-8315) as the standard
- A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
- A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
- IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
- IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
- On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
- The biological activity of the IFN gamma homodimer is highly species specific
- Human IFN gamma does not show cross-reactivity with mouse
- IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
- IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
- Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
- IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
- IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
- IFN gamma gene mutations are associated with aplastic anemia.
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Antigen: IFN gamma
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P01581
Gene Symbols: IFNG
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 25712
Content And Storage: 4° C
Form: Liquid
Applications: ELISA
Clone: DB-1 (DB1)
Conjugate: Unconjugated
Gene: IFNG
Gene Alias: BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
Host Species: Mouse
Quantity: 500 μg
Primary or Secondary: Primary
Target Species: Rat
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
IFN gamma
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P01581
Gene Symbols:
IFNG
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
25712
Content And Storage:
4° C
Form:
Liquid
Applications:
ELISA
Clone:
DB-1 (DB1)
Conjugate:
Unconjugated
Gene:
IFNG
Gene Alias:
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
Host Species:
Mouse
Quantity:
500 μg
Primary or Secondary:
Primary
Target Species:
Rat
Product Type:
Antibody
Isotype:
IgG1 κ
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