IFN gamma Monoclonal Antibody (NIB42), eBioscience™, Invitrogen™

Description

• Description: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma)
• The NIB42 antibody is a neutralizing antibody
• IFN-gamma is an ∽20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine
• IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells
• Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma
• Glycosylation of IFN-gamma does not affect its biological activity
• Applications Reported: The NIB42 antibody has been reported for use in capture of human IFN gamma by ELISA, and for neutralizing IFN gamma bioactivity
• Use Functional Grade purified for functional assays cat
• 16-7318)
• Applications Tested: The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 (13-7319) antibody for detection and recombit human IFNg (29-8319) as the standard
• A suitable range of concentrations of this antibody for ELISA capture is 2-8 μg/mL
• A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.For ELISPOT Capture, the alternative clone MD-1 is recommended
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
• IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
• IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
• On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
• The biological activity of the IFN gamma homodimer is highly species specific
• Human IFN gamma does not show cross-reactivity with mouse
• IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
• IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
• Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
• IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
• IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
• IFN gamma gene mutations are associated with aplastic anemia.