50-137-45

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eBioscience™, Invitrogen™

Manufacturer: Invitrogen

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Pack Size SKU Availability Price
Each of 1 50-137-45-Each-of-1 In Stock ₹ 14,117.40

50-137-45 - Each of 1

₹ 14,117.40

In Stock

Quantity

1

Base Price: ₹ 14,117.40

GST (18%): ₹ 2,541.132

Total Price: ₹ 16,658.532

Antigen

SSEA4

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Host Species

Mouse

Quantity

25 μg

Primary or Secondary

Primary

Content And Storage

4° C

Form

Liquid

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Clone

eBioMC-813-70 (MC-813-70)

Conjugate

Unconjugated

Gene Alias

SSEA-4; stage-specific embryonic antigen 4

Purification Method

Affinity chromatography

Regulatory Status

RUO

Target Species

Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit

Product Type

Antibody

Isotype

IgG3

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Description

  • Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein
  • Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation
  • Recently expression has also been found on bone marrow mesenchymal stem cells
  • The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys
  • Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry
  • Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by flow cytometric analysis of the 2102Ep cell line
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Purity: Greater than 90%, as determined by SDS-PAGE
  • Aggregation: Less than 10%, as determined by HPLC
  • Filtration: 0.2 μm post-manufacturing filtered
  • SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS)
  • In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation
  • Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

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Invitrogen

50-137-45

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Antigen:
SSEA4

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Host Species:
Mouse

Quantity:
25 μg

Primary or Secondary:
Primary

Content And Storage:
4° C

Form:
Liquid

Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Clone:
eBioMC-813-70 (MC-813-70)

Conjugate:
Unconjugated

Gene Alias:
SSEA-4; stage-specific embryonic antigen 4

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Target Species:
Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit

Product Type:
Antibody

Isotype:
IgG3

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