CD282 (TLR2) Monoclonal Antibody (6C2), eBioscience™, Invitrogen™

Description

• Description: The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2)
• Mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line
• To date, at least twelve members of the Toll family have been identified in human and mouse
• This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor
• Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation
• TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens
• Applications Reported: This 6C2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections
• Unpublished preliminary studies indicate that this antibody may inhibit some aspects of monocyte activation through TLR2, however, it may not result in complete blocking of TLR2 function
• For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, cat
• 14-0161)
• Applications Tested: The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocytes or Raw264.7 cells
• TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity
• TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B
• TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively
• TLRs are highly conserved from Drosophila to humans and share structural and functional similarities
• The various TLRs exhibit different patterns of expression
• TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB
• TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity
• Ten human homologs of TLRs (TLR1-10) have been described
• TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers
• CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells
• The expression of TLR2 on different cell types are regulated by different immune response modifiers
• For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.