50-140-42

CD33 Monoclonal Antibody (HIM3-4), PE-Cyanine5, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

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Pack Size SKU Availability Price
Each of 1 50-140-42-Each-of-1 In Stock ₹ 28,748.16

50-140-42 - Each of 1

₹ 28,748.16

In Stock

Quantity

1

Base Price: ₹ 28,748.16

GST (18%): ₹ 5,174.669

Total Price: ₹ 33,922.829

Antigen

CD33

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P20138

Gene Symbols

CD33

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

945

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

HIM3-4

Conjugate

PE-Cyanine5

Gene

CD33

Gene Alias

CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family
  • CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes
  • Hematopoietic stem cells and lymphocytes do not express this antigen
  • CD33 plays a role in sialic-acid dependent cell adhesion
  • Applications Reported: The HIM3-4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This HIM3-4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (1 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
  • CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family
  • It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface
  • CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia
  • Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells
  • CD33 is also expressed on, and is a useful marker for, peripheral monocytes
  • CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias
  • Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

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Invitrogen

50-140-42

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Antigen:
CD33

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P20138

Gene Symbols:
CD33

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
945

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
HIM3-4

Conjugate:
PE-Cyanine5

Gene:
CD33

Gene Alias:
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

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Invitrogen

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Antigen:
CD38

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P56528

Gene Symbols:
Cd38

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
12494

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
90

Conjugate:
PE-Cyanine5

Gene:
Cd38

Gene Alias:
T10, ADP-ribosyl cyclase 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase, CD38 antigen p45, NAD+ nucleosidase, cADPr hydrolase 1, cyclic ADP-ribose hydrolase 2

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

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Invitrogen

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Antigen:
CD38

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P28907

Gene Symbols:
CD38

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
952

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
HIT2

Conjugate:
PE-Cyanine5

Gene:
CD38

Gene Alias:
2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; Cd38; CD38 antigen; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase); CD38 antigen (p45); CD38 antigen p45; CD38 molecule; CD38H; Cd38-rs1; cluster of differentiation 38; cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; I-19; NAD(+) nucleosidase; NAD+ nucleosidase; NIM-R5 antigen; T10

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

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50-140-48

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Antigen:
CD40

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P27512

Gene Symbols:
CD40

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
21939

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
1C10

Conjugate:
PE-Cyanine5

Gene:
CD40

Gene Alias:
AI326936; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; Cd40; CD40 antigen; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen, TNF receptor superfamily member 5; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; GP39; HIGM1; I79_006806; IGM; IMD3; Immunoglobulin M; ImmunoglobulinM; membrane protein CD40; MGC9013; p50; receptor for ligand CD154; sCD40; soluble CD40; T-BAM; T-cell differentiation antigen; Tnfrsf5; TRAP; tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ