CD14 Monoclonal Antibody (61D3), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein
• CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells
• Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14
• Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis
• 61D3 has also been reported for in vitro functional studies
• (Fluorochrome-conjugated 61D3 is recommended for use in flow cytometry)
• Applications Tested: The 61D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils
• CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form
• CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein
• The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles
• Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes
• Alternative splicing results in multiple transcript variants encoding the same CD14 isoform
• Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.