CD70 Monoclonal Antibody (FR70), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein
• CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes
• Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation
• Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis
• FR70 has also been reported in blocking of CD70 in vitro
• Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family
• This cytokine is a ligand for TNFRSF27/CD27
• It is a surface antigen on activated, but not on resting, T and B lymphocytes
• It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation
• This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.