DLL1 (delta-like 1) Monoclonal Antibody (HMD1-5), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The HMD1-5 monoclonal antibody reacts with mouse Delta-like 1, one of five type I transmembrane proteins that serves as a Notch receptor ligand
• Upon binding the Notch receptor (e.g
• Notch 1-4), Delta-like 1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase
• The second cleavage event releases an intracellular fragment whose biological function remains controversial
• Delta-like 1 is expressed by thymic stromal cells, as well as on macrophages, dendritic cells, and stromal cells in the spleen
• This protein is also expressed in nonhematopoietic tissues such as the lung and brain
• Delta-like 1 has been linked to B cell development and differentiation, especially marginal zone and plasma cells, regulation of splenic dendritic cells, and leukemogenesis
• This monoclonal antibody has been reported to have neutralizing activity
• Applications Reported: This HMD1-5 antibody has been reported for use in functional assays
• Applications Tested: This HMD1-5 antibody has been tested by flow cytometric analysis of Delta-like 1-transfected CHO cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Delta-like protein 1 (DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats
• It is a ligand for Notch receptor
• It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.